This paper deals with the study of a new detailed analysis and realization method of spectrum based on labVIEW, by using labVIEW’s powerful mathematical and signal analysis function on the basis of the traditional polyphony refining analysis method, which provides a new way for realize the detailed analysis of frequency in the virtual instrument by means of the software platform-labVIEW. The simulation and engineering example shows that the method can meet the engineering requirements for the signal frequency’s refining analysis.