The essential oil from the leaf of Jasminum mesnyi, collected from Kirtipur, Kathmandu, Nepal, was obtained by hydrodistillation and analyzed by GC-MS. From a total of 31 peaks, 25 compounds were identified in the oil, accounting for 91.1% of the oil. The majority of the essential oil was dominated by the benzopyrone coumarin (48.9%). The oil also contained major amounts of monoterpenols including linalool (14.8%), α-terpineol (5.2%), and geraniol (3.3%). Other components of the essential oil found in smaller proportion included (Z)-asarone (3.5%) and (E)-phytol (3.4%). The oil was screened for antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Aspergillus niger, but showed no appreciable activity (MIC ³ 1250 μg/mL). Jasminum mesnyi oil did show marginal activity in the brine shrimp lethality test (LC50 = 27.03 μg/mL).