Diabetes mellitus (DM) has affected 7% of the world’s adult population. Spirulina fusiformis is a rich nutritional supplement which is non-toxic and has antidiabetic, antioxidant and hypolipidaemic activity. Our study was aimed to investigate the in silico inhibitory effect of the chosen active compounds of Spirulina(3Z-phycocyanobilin, β- carotene and Vitamin B12) against human xanthine oxidoreductase, human glycogen synthase kinase 3-β, α- glucosidase, human aldose reductase and α-amylase. The docking experiments were carried out using Patch Dock server. PyMol molecular viewer was used for the analysis of patterns of interaction of the ligands against the chosen receptors. Vitamin B12 revealed significant interactions showing six hydrogen bonds each with human xanthine oxidoreductase and human glycogen synthase kinase 3-β.The potential of vitamin B12 in this regard needs to be studied further by appropriate in vivo models.