Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2022 Vol: 14 Issue: 9

Validated UV-Spectrophotometric Method for Simultaneous Estimation of Benidipine Hydrochloride and Telmisartan in Bulk and Pharmaceutical Dosage Form

Abstract

A simple, economical, accurate, precise and less time consuming UV spectrophotometric method has been developed and validated for simultaneous estimation of Benidipine hydrochloride and Telmisartan in bulk and pharmaceutical dosage form. In this method Benidipine HCl and Telmisartan are exhibits maximum absorbance (λmax) at 237 nm and 296 nm with methanol as the solvent. The method was validated as per the International Conference on Harmonization (ICHQ2R1) guidelines. Drugs followed the linearity in the concentration range of 5-25 μg/mL and 2- 10 μg/mL with correlation coefficient (r2) of 0.999 for Benidipine HCl and Telmisartan respectively. The validity of the proposed method was assessed by applying the standard addition technique where the percentage recovery of the added standard was found to be 98.68 and 99.6 for Benidipine HCl and Telmisartan. The limit of detection and quantification were calculated and found to be 1.529 µg/mL and 4.634 μg/mL and 0.457 µg/mL and 1.386 µg/mL for Benidipine HCl and Telmisartan respectively. The proposed method is recommended for routine analysis of Benidipine hydrochloride and Telmisartan in bulk and pharmaceutical dosage forms in regular quality control testing laboratories.

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