Two simple, accurate and reproducible spectrophotometric methods have been developed and validated for simultaneous estimation of glimepiride and metformin in combined dosage form. Glimepiride shows maximum absorbance at 222 nm and metformin at 228 nm. First method was based on first-order derivative spectroscopy, in which wavelengths selected were 249 nm (zero crossing of metformin) where glimepiride shows considerable absorbance and 258 nm (zero crossing of glimepiride) where metformin shows considerable absorbance. The second method was based on multicomponent mode technique, in which sampling wavelengths selected were 222 and 228 nm. Linearity for detector response was observed in the concentration range of 3-15 μg/mL for glimepiride and 1.0-5.0 μg/mL for metformin for method I; 2.0-10.0 μg/mL for glimepiride and 0.5-2.5 μg/mL for metformin for method II respectively. Accuracy and precision studies were carried out and results were satisfactory. The proposed methods were validated as per ICH guidelines. The developed methods are simple, precise, rugged and economical. The utility of the methods has been demonstrated by analysis of commercially available formulations.