Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2018 Vol: 10 Issue: 5

UV Spectrophotometric Method for Simultaneous Estimation of Glycopyrrolate and Formoterol Fumarate in Their Synthetic Mixture by Absorbance Correction Method

Abstract

A simple, precise, accurate and validated UV-spectroscopic method absorbance correction menthod for simultaneous estimation of glycopyrrolate and formoterol fumarate in their synthetic mixture was developed. The Estimation was done based on measurement of absorbance at two wavelengths 295 nm(λ1) and 209 nm(λ2). Were λ1 was the point at which Formoterol Fumrate showed significant absorbance and Glycopyrrolate showed zero absorbance and λ2wavelength at which both drugs showed significant absorbance. Linearity was obtained over a range of 2-10 µg/ml for glycopyrrolate and 1-3 µg/ml for Formoterol Fumarate respectively. The results of proposed method were validated for linearity, precision, accuracy, robustness, ruggedness, LOD & LOQ. This method can be successfully be applied for simultaneous estimation of drugs in all commercial products.

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