The objective of the study was to develop simple, accurate, precise and rapid UV first and second order derivative spectrophotometric methods with subsequent validation by using ICH guidelines for the determination of ofloxacin in pharmaceutical dosage form. The proposed first and second order derivative methods involve the measurement of absorbance of drug at 278 nm and 234.6 nm for the estimation of ofloxacin respectively. The linearity of the proposed methods were found in the concentration range of 0.5 – 10 μg /ml (r2= 0.9995) for first order and 2 – 10 μg /ml (r2= 0.9953) for second order derivative methods and the percentage mean recovery was found to be 100.069 % and 99.792% respectively. The methods were also statistically validated for its linearity, accuracy and precision. Both intra and inter day variation showed less percentage (%) RSD values indicating high grade of precision of these methods.