Density (d) and ultrasonic velocity (v) of 2-hydroxy substituted quinoxaline in dioxane were measured at 305.85 K temperature by using ultrasonic interferometer at a frequency of 1 MHz. The study was carried out with change in concentrations. Various thermodynamic parameters such as adiabatic compressibility (), intermolecular free length (Lf), specific acoustic impedance (Z) and relative association (RA) were calculated. The results were used to discuss the interaction between solute and solvent. The results are interpreted in terms of molecular interactions like solvent-solvent, solvent-solute and solute-solute.