A U.V. spectrophotometric method is developed for the quantitative estimation of Keterolac Tromethamine in pharmaceutical dosage forms. The present method is simple, accurate, economical and reproducible for the estimation of Keterolac Tromethamine in pharmaceutical dosage forms. The solutions are prepared in acidic methanol solvent. Maximum absorbance is observed at 316 nm and measurements were made at 316 nm wavelength. Beer’s law is obeyed and linearity is observed in the concentration range of 2.5 – 15 mg/mL. The correlation coefficient is found to be 0.9999. The % recovery was 99.23 – 99.48%. This method is validated as per ICH guidelines and the results were with in the limits. The present U.V. Spectrophotometric method is accurate, simple, sensitive, economical and reproducible for the estimation of Keterolac in pharmaceutical dosage forms.