This study was carried out to analyze Physicochemical parameters like Colour, odour, pH, electrical Conductivity (EC),Total suspended solids (TSS), Total dissolved Solids (TDS), Dissolved Oxygen (DO), Biochemical Oxygen Demand(BOD), Chemical Oxygen Demand (COD), Hardness, Sulfatesand Chloride of untreated sugar mill effluent and to degrade the effluent using biocontrol agents Trichoderma harzianum and Pseudomonas fluorescens. In the present study the effect of effluent (uninoculated) on seed germination at various concentrations and growth (inoculated) of chick pea was studied. The results of the parameters analysis revealed that untreated sugar mill effluent was green black in colour with offensive odour. pH was low with high organic load such as EC, TSS,TDS, BOD and COD which were higher than the permissible limit. The results of the degradation study shows that fungus Trichoderma harzianum and bacterium Pseudomonas fluorescens were found to be very much effective in reduction of pollutants (selected parameters). Decolorization results also reveal reduction color intensity with the incubation time. Trichoderma harzianum was more effect at the reduction percentage range of 20-88% whereas Pseudomonas fluorescens showed reduction percentage range of 16-8 %. Germination studies was conducted with chick pea seed treated with different concentrations (control, 10,25,50,75 and 100% ) of sugar mill effluent. Germination decreased with the increase of effluent concentrations (25-100%).Growth parameters such as shoot length, root length,dry weight of seedlings were found to be increased in comparision to control.