Laportea aestuans (Linn.) Chew as original plant from Papua has been using for pain relief as traditional medication in the local community widely. The objective of this study aims to screen active compound chemical, determine LC50, IC50, and analgesic-antiinflamatory activity effect of ethanolic extract from L. aestuans (ELA). Sample that picked up from Biak Indonesia was determined in Herbarium Manokwariensis. Ethanolic extract was tested the toxicity with BSLT and antioxidant effect by DPPH methode. Then ELA was evaluated analgesic with tail flick test and anti-inflammatory effect with carragen-induced paw edema with 0.3 g/kg, 0.6g/kg, and 1.2g/kg dose on white male rat. This study resulted that ethanolic extract Laportea aestuans (Linn) Chew positively contained alkaloids, flavonoids, saponins, and tannins. The extract was toxic with LC50 285 μg/mL and weak antioxidant activity with IC50 of ELA had 554.30 μg/ml. According to tail flick methods, dose maximum of ELA was 1.2g/kg resulted percent analgesic effect 54.70% and 0.6g/kg presented 39.62% antiinflamantory activity.