Plants have been one the most important sources for producing medicines since ancient times. In this study, antioxidant and antibacterial activity of Punica granatum was analysed using TLC-bioautography analysis. The plant leaves were collected and subjected for crude extraction using several solvents. The crude extracts were analysed for phytochemicals followed with antibacterial and antioxidant activity by TLC bioautography assay. The extract which showed a promising result were subjected for column fractionation and the collected fractions were subjected for spot assay for antibacterial and antioxidant activity. The plant was found to possess antioxidant and antibacterial activity.