The antibacterial activity of Garcinia kola seeds extracts against Staphyloccoccus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumonia of was investigated. Garcinia kola Seeds were purchased from Okitipupa local market in okitipupa metropolis of Ondo State. The seeds were dehusked, chopped, air dried, pulverized and stored in a clean air tight bottle sample. 100 g of pulverized Garcinia kola seed was extracted with 900 mL of analytical grade methanol. The mixture was filtered and concentrated to obtain a crude extract. Agar-gel diffusion inhibition technique was adopted for the antimicrobial screening of Garcinia kola seed. 0.2 ml of 50 mg/ml, 75 mg/ml and l00 mg/ml of Garcinia kola seed extract were gently poured into each of the wells in the agar plates. Agar plates of ampiclox were also prepared as control. The agar plates of both extracts and antibiotic were then incubated at a temperature of 37°C for 24 hours. The zones of inhibition around the well were measured. The zones of inhibition of the extract against selected bacteria range from 00 mm to 21 mm. The control (ampilox) showed zones of inhibition of 22, 30, 32 and 29 mm against Staphyloccoccus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumonia respectively. Based on the zones of inhibition the order of susceptibility of the bacterial to the extract was Staphyloccoccus aureus>Escherichia coli>Bacillus subtilis>Klebsiella pneumoniae. The results obtained from this study, provides scientific evidence that can help in validation of Garcinia kola seed as antibacterial agent for the treatment of bacterial infections. Also, the insilico docking study showed that kolaviron have the highest tendency to inhibit all the studied bacterial cell lines.