The synthetic Hydroxyapatite (HAp) nanoparticles are extensively used to significantly improve the biocompatibility and bioactivity of man-made biomaterials. A wide variety of methods are available for the preparation of HAp nanoparticles, choosing a procedure to synthesize it for specific requirement is laborious. Hence, in our present investigation we try to evaluate the Hydroxyapatite crystals synthesized in four different methodologies. The methodologies used to synthesis the hydroxyapatite nanoparticles via hydrothermal, sol-gel, precipitation and microwave methods. The calcium and phosphorus precursors were chosen with molar ratio of 1: 0.6 to achieve the synthetic bone like apatite. The prepared samples with different synthetic methods were characterized using XRD, FE-SEM, EDAX and Raman to ascertain the crystalline size, phase structure, morphology, stoichiometric ratio and the functional groups present in the resultant hydroxyapatite nanoparticles.