We aimed to study the effects of alcoholic extract of Eryngium billardieri on lipid profiles levels and liver function tests in hypercholesterolemic rats. In this study, 35 male wistar rats in 5 groups (n =7) were selected: Control group with normal diet, sham group with fat diet, and three experimental groups containing animals with fat diet that daily received a minimum dose of 100 mg/kg ,an average dose of 200mg/kg and a maximum dose of 300mg/kg of hydro alcoholic extract of Eryngium billardieri over a period of 21 days, respectively by gavage feeding. Amounts of lipid and lipoprotein profiles, with low and high density, as well as indicators of liver function such as alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), albumin, and Serum total protein and renal function tests, such as creatinine and blood urea nitrogen (BUN), in blood samples were taken. Results Serum ALT and ALP in the experimental group receiving the minimum dose of the extract were significantly reduced compared to the sham group (P ≤0.05). The groups which received the extract showed significant decrease in cholesterol, triglycerides and LDL. While in all groups receiving the extract no significant changes in BUN and creatinine were seen compared to the sham group. Conclusions: These results suggest that the extract of Eryngium billardieri which has alkaloid and antioxidant compounds can improve liver function in hypercholesterolaemic rats without leaving side effects on their renal functions.