The big five model of personality has been considered by many psychologists as a popular and powerful approach for the study of personality characteristics. These include neuroticism, extraversion, openness, agreeableness, and conscientiousness. Recognition of blood types could help in the recognition personality traits. This is a descriptive and correlation study. 400 people were enrolled in the study using the convenience sampling method. The 60-item NEO-PI was used for data collection. Data entered into SPSS version 18 and analyzed using descriptive statistics including frequency distribution tables, mean, standard deviation, Kolmogorov-Smirnov test, one-way ANOVA and post hoc LSD tests. Mean of neuroticism in blood type O and blood type B had the highest (23.83) and lowest scores (22.28) respectively. Mean of extraversion in blood type A and blood type B had the highest (30.76) and the lowest score (28.67) respectively. Mean of openness in blood type A and blood type AB had the highest (25.72) and the lowest score (23.93) respectively. Means of agreeableness and conscientiousness in blood type A had the highest scores of 30.16 and 35.47 respectively. In this study, personality factors of openness and extraversion were associated with different blood types (p = 0.04). From the five-factor personality traits, the three factors of Neuroticism, Conscientiousness and Agreeableness showed no relationship with blood types; however, both Extraversion and Openness were linked with different blood types.