Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 10

The Pharmacognostical Evaluation of the Marrubium vulgare Linn Collected from the Pulwama District of Jammu and Kashmir State in India

Abstract

The objective of the present research work was to establish the various pharmacognostical parameters for the Marrubium vulgare Linn (Family- Lamiaceae) collected from the Pulwama district of Jammu and Kashmir region of India. The evaluation of organoleptic, microscopical features (root, stem and leaf) and leaf constant such as vein islet number, vein termination number, stomatal index and palisade ratio aids in detection of adulteration and allied species of the plant. The study of various physicochemical parameters helps in determining the quality of the herb. The higher alcohol soluble extractive value (8.66 ± 1.2%) suggests that most of the constituents of the plant were soluble in alcohol. The total ash acid insoluble ash and water soluble ash was found to be 10.7 ± 0.46, 1.73 ± 0.61, 8.9 ± 0.65 % respectively. The moisture content and total fibre content of the drug was 17.2 ± 0.35 % and 9.5 ± 0.88 % respectively. The plant was also extracted by percolation and microwave assisted method of extraction and the yield of the extract was almost double, from 11.27 ± 1.2 in percolation method to 20.23 ± 1.91% w/w in novel technique. The preliminary phytochemical screening of the extracts confirmed the presence of alkaloids, terpenoids, steroids, flavonoids and carbohydrates in both the extracts. The quantitative estimation of two extracts revealed that the microwave technique significantly increase the total phenolic content from 61.44 ± 2.01 to 93.42 ± 1.04 mg of GAE/gm of extract and total flavonoid content to 37.7 ± 1.66 from 23.25 ± 0.94 mg of RUE/gm of extract as compared to extract obtained by conventional method.

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