Diseases in fish could cause a decrease in fish productivity could even lead to the death of fish up to 90% of the total population. Fish disease is anything that can cause disruption in fish, either directly or indirectly, and these disorders are caused other organisms, feed or environmental conditions that do not support fish life. The objective of this study was to determine the doses that effective for inactivating streptococcosis on tilapia. Induction period of the vaccine and challenge test of clinic symptoms and determine the survival rate of tilapia after challenge test. Methods used in this study is completely randomized design (CRD) with three treatments. The use of the vaccine treatment from bacterial isolat O at a density of 107 cfu. From density of 107cfu vaccine divided into three different doses of the vaccineis: vaccine at a dose of 0.75 ml, vaccine at a doses of 1.25 ml, and vaccine at a dose of 1.75 ml. Parameter swereob served survival rate and clinic symptoms of tilapia. Here, we showed that the vaccine of the bacteria Streptococcus iniae with 107 cfu density at a dose of 1.75 ml inactivated by heat kill the density of the most effective and efficient in triggering immunity tilapia against bacteria Streptococcus iniae with the survival rate of 90%. This study provides a new channel of research in making vaccines fish diseases in freshwater fishery more efficient.