Recently concerns were raised about the adverse effect of the leached monomers, from the resin-based materials, particularly bisphenol-A (BPA) because of its demonstrated estrogenic effect. Three commercially available bisphenol-A glycidyl methacrylate (Bis-GMA) resin-based restorative materials were used in this study. These products were two pit and fissure sealants Fisseal and ClinproTM. and one resin composite restorative materials FiltekTM Z 350XT flowable restorative. The extracted immersion media were analyzed using HPLC for the detection of the leached out BPA. The leached components were administered intragastrically daily to the test groups and the equivalent volumes of the immersion media were administered to the control groups of mice for one week, two weeks and three months. At the end of each experimental period, blood samples were withdrawn from retro-orbital plexus of all mice. The collected serum samples were tested for the activity of estrogen receptor using Mouse Estrogen Receptor Alpha (ERα) ELISA kit. Then all animals were sacrificed and ovaries from females as well as testes from male mice were dissected and fixed in formalin saline 10% for histological examination. The results revealed that flowable composite immersed in citric acid showed higher BPA monomer release and higher values of estrogen receptor activity than both sealants. Higher values of estrogen receptor activity were observed for female mice and citric acid subgroups. Ovaries of female mice were more affected than testes of males and these affections ranged from increased proliferation of the interstitial stromal cells in the medulla to severe congested ovarian blood vessels.