This study aimed to find out the effectiveness Atonik and Rootone F to the growth response stem cutting of Rosa sp. The method used was experimental method completely randomized factorial design, with four replications. The first factor was the growth regulator atonik that given to the level of concentration of 0.0 to 3.0 ml/L. The second factor was the growth regulator Rootone F with a concentration of 2.5 mg. Result showed at the first week after planting, stem cuttings of roses had shown the shoot growth response. At the fifth weeks, all aspects of vegetative growth on stem cuttings of roses reached maximum growth. Concentration of 3.0 ml/L Atonik could increase the maximum growth of stem cuttings of roses, until at the fifth weeks could reached an average of 3 shoots, 5.6 cm of long shoot, 5 petioles per bud, 5 leaves per petiole, 3.3 cm of long petiole, 4 cm of long leaves, 7.5 cm of long root, and 26 roots. Treatment of 3.0 ml/L atonik effective in improving vegetative growth on stem cuttings of Rosa sp