Insulin resistance is a metabolic disorder which alters the liver inducing de nova lipogenesis and glucogenesis. The aim of the present study is to evaluate the chronic administration of Withania somnifera Root powder (WS) on blood sugar, lipid profile, and liver enzymes in fructose drinking water (FDW) rats. At the beginning ,48 Wistar-Albino male rats, weighting 200±20 g were divided into control( C) and (FDW) group(n=24): half of C and FDW groups received 62.5mg/g diet WS root powder(CWS & FWS groups) during trial period(n=12). Group C did not receive any agent but group FDW received fructose-enriched water (10%, w/v) during trial period. At the end of the experiment, animals were anesthetized by diethyl ether, sacrificed and blood samples were collected from cervical vessels. Serum insulin, blood glucose, lipid profile and liver enzymes were measured. Obtained data were analyzed by SPSS Software Ver. 18; using ANOVA and tukey test. Results were expressed as mean ± SD. Statistical differences were considered significant at p<0.05. The results of the present study showed that Blood glucose, serum insulin, insulin resistance index, triglyceride, and AST and ALT in group FDW were significantly higher than groups C and CWS but this values in group FWS decreased significantly compared to FDW group (p<0.05). Our results indicated that chronic administration of witania somnifera root powder in diet improved blood glucose, serum insulin, insulin resistance index and liver enzymes in FDW rats.