Leishmania tropica is the causative agent of cutaneous Leishmaniasis in mammalian. Previous studies showed that vaccination Balb\c mice with inhibited Leishmania tropica by mitomycine\c induced production of interferon gamma in the serum of mice, but does not protect it from developing a lesion where it was mixture of Th1 and Th2. To enhance immune response, we evaluate the role of the genomic DNA of Leishmania tropica as adjuvant in combination with inhibited Leishmania tropica promastigotes by mitomycine\c in the immune response as a vaccine by inoculation the right footpads of Balb\c mice. After 4 weeks from vaccination, the results showed absence of any dermal lesions, absence of parasites in the inoculation site, high gene expression of interleukin -12, and interferon gamma, low gene expression of interleukin -4 in the draining lymph nodes of the vaccinated footpads. These results indicate that the dominant immune response it could be Th1.