Many species of Asteraceae are used as medicinal plants with different therapeutic indications, preparation and uses. The species Acmella ciliata (Kunth.) Cass belongs to this family. It is a medicinal plant of African origin, brought to Brazil by slaves during the colonial period. The extract from the leaves of A. ciliata (Kunth.) Cass has several uses in Brazilian folk medicine, since it has analgesic and anti-inflammatory properties. Thus, this research aims to identify the chemical compounds present in the aqueous crude extract of leaves of A. ciliata (Kunth.) Cass (Asteraceae) evaluate the antioxidant potential against the DPPH radical and cytotoxic front the Larvae of Artemia Salina Leach. Evaluation of antioxidant activity, with some modifications, was based on the methodology proposed by Sousa and Lopez-Lutz by sequestering capacity of DPPH• (2,2-diphenyl-1-picryl-hidrazila). The cytotoxicity assay front Artemia salina Leach. based on the technique of Araujo and Lôbo with adaptations. In this study, the preliminary phytochemical analysis of EBA A. ciliata (Kunth.) Cass. revealed the presence of saponins, organic acids, reducing sugars, tannins, alkaloids, steroids and triterpenoids. Based on these results it is concluded that the EBA leaves A. ciliata (Kunth.) Cass has in its chemical constitution promising secondary metabolites with antioxidant action. Cytotoxic activity was not significant considered non-toxic in this test. However, it is evident the great therapeutic potential of this species.