The present study was to compare the effect of passion flower’s extract and fluoxetine on depression symptoms in mice using Tail suspension test. In this empirical study, number of 50 mature mice (weight range of 25 to 30 gr) was used in five groups. Depression symptoms were observed in four groups, after injection of 25 mg/ kg tetrabenazine. Three groups has been received 50, 100 and 200 mg/ kg hydro alcoholic extract of passion flowers as for rat's weight via intraperitoneal injection. Group four has been received 1.4 mg/kg fluoxetine, while group five as control ones received no treatment. The Tail suspension test was used to assess depression mood, and increase in the movement time to second and decrease of time to moveless assigned as an index for depression. The SPSS software was used for analyzing collected data. Compare to control group and fluoxetine, hydro alcoholic extract of passion flower’s with concentration of 50 and 100 mg/kg of mice weight has been significantly increased time of movement and decreased time of moveless in samples; and these all shows depression mood. The results of this study suggest that 50 and 100 mg/kg hydro alcoholic extract of passion flower can be an appropriate alternative for fluoxetine to decrease depression symptoms (p˂0.05).