Patients with diabetes type 2 have a higher risk of atherosclerosis disease. In addition to insulin resistance which results in growth and differentiation failure of osteoblasts, leading to failure in Osteocalcin secretion. This study is carried out to investigate the association of serum Osteocalcin with atherosclerosis in patients with type 2diabetes and in patients with atherosclerosis without diabetes. Serum Osteocalcin was measured by ELIZA method, Fasting blood glucose, LDL, total cholesterol, TG were measured by enzymatic colorimetric method in 65 subjects.(20 atherosclerotic but non diabetic patients;24 diabetic type 2 atherosclrrotic patients and 21 apparently healthy subjects(control group). The mean serum osteocalcin concentration for diabetic type 2 atherosclrrotic patients and atherosclerotic patients without diabetes was 13.483±3.325 ng/ml,20.516±3.481ng/ml, respectively. Osteocalcin levels were associated inversely with Fasting serum glucose, LDL, TG, Tcho (P<0.05). As a Conclusions, Low Serum Osteocalcin levels are significantly associated with atherosclerosis.