In this study, the exhaust analyzer (EA) and process mass spectrometer (PMS) were employed to investigate the influence of Human-like collagen (HLC) production. As a result, the biomass and HLC production had an obvious increase by the PMS. Using this measuring method the final optical density and HLC concentration were 152 and 8.02 g/L, respectively. Additionally, the concentration of acetate in the medium was at a lower level compared with that by the EA. The PMS was quickly and accurately to the feedback of the dissolved oxygen. Hence, the result showed that the cells were cultivated in a relative stabilized environment by the PMS, which could harvested a high level of HLC and cell density.