A series of Schiff base transition metal complexes of general formula [MLaLb] (wher M = Co(II), Ni(II), Cu(II), Zn(II), and Cd(II); La = Schiff base ligand derived from o-hydroxyacetophenone and L-valine; Lb = 4,4’-bipyridyl) have been synthesized and characterized on the basis of elemental analyses, molar conductance and spectroscopic studies such as UV-Visible, FTIR and ESR. A square planar geometry has been assigned based on the analyses. In vitro antibacterial and antifungal activities of Schiff base and metal complexes have been screened against four bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus sps. and Escherichia coli) and three fungal strains (Aspergillus flavus, Rhizophus sps. and Mucor sps.) by the agar-well diffusion method. The results revealed that the synthesized metal complexes exhibit good antimicrobial activity. In addition, the antioxidant and larvicidal activities were also carried out. The larvicidal activity against Culex quinquefasciatus emphasized that the metal complexes were more potent than the free Schiff base ligand.