Synthon disconnection strategy is a problem solving technique in the planning of organic syntheses. This strategy, developed by Prof. E.J.Corey of Harvard University, plays vital role in the synthesis design of many bioactive natural products used in analytical biochemistry, molecular biology and drug discovery process. Keeping an overview on the published works in both journals and patent literatures, a good number of synthesis schemes have been proposed based on this strategy for a bioluminescent natural product ‘Coelenterazine’ isolated from marine organism jellyfish Aequorea victoria that finds extensive applications in bioassays. The proposed synthesis planning being a theoretical investigation, the actual laboratory execution requires the cross examination of a considerable number of factors such as reactions, reagents and order of events. In actual practice, generally that route is most feasible which is cost-effective, safe, and easy to carry out and gives maximum yield in a short reaction time.