In this paper, a number of new derivatives N-phenyl-2-chloro-9H-pyrido[2,3-b]indole-3-ylmethanimine were synthesized using of the Vilsmeier–Haack reagent (DMF+ POCl3). To this end ,the initial composition of 1H-2- indolamine after the acquisition of a group methyl- ketone (1) , gave the fused pyridine ring by cyclization and a free aldehyde group is made (2) . Finally , after using aryl-amine derivatives one type, made imine bond connection to the 2-chloro- 9H-pyrido[2,3-b]indole (3) . final products were supported by FTIR , 1H NMR and Mass spectroscopic measurements and possess moderate to good antifungal and antibacterial activities.