Selective molecularly imprinted polymers (MIPs) for solid-phase extraction and determination of glipizide have been designed and prepared. MIPs were synthesized with acrylamide as the functional monomer and ethyleneglycoldimethacrylate as the cross-linker in chloroform as a porogenic solvent by using bulk polymerization method. MIPs are able to bind 100% glipizide in chloroform by using a batch method compared to a non-imprinted one. By using Fourier Transform Infra Red, it was found that hydrogen bonding occurred between the carbonyl group of acrylamide and the proton donor of template. These results find the opportunity for this MIP to be used as an alternative of the pretreatment method before determination of glipizide by using glibenclamide as a pseudotemplate.