Thienopyrimidines forms a significant class of drugs which exhibit an array of biological activities ranging from anti-inflammatory, antibacterial, antifungal, analgesic, antiviral to anticancer activity. In continuation of our research program on thienopyrimidines, the present study focused on synthesizing the thienopyrimidines derivatives possessing different heterocyclic rings to obtain highly potent anti-inflammatory agents. Thienopyrimidines have been synthesized by the cyclisation of thiophene with formamide to obtain thieno [2, 3-d] pyrimidine. The synthesized compounds were screened for their anti-inflammatory activity using bovine serum albumin denaturation (in vitro) assay model, by using ibuprofen as a standard. The present study has given deep insight as the thienopyrimidines bearing pyrazole, oxadiazole and thiadiazole rings shown significant anti-inflammatory activity. The compound (8a) containing thiadiazole was found to be most potent.