Synthesised N- glycosyl amide molecules of fatty acids with carbon chain lengths varying from C6 to C18 via the modified Staudinger reaction. Biosurfactant properties of these non-ionic compounds were determined along with their antifungal activity. The glucolinoleiamide (5e) and maltolinoleiamide (5f) have exhibited good surface tension with 37 mN/m and 43 mN/m respectively which are comparable with that of rhamnolipids. Compound 5e also showed very good fungicidal activity against Sclerotium rolfsii (ATCC 62666) while maltolinoleiamide (5f) and glucohexanoylamide (5d) exhibited inhibitory activity against Mucor indicus (MTCC 7135).