Six compounds (5a-5f) containing 1,3,4-thiadiazole and Schiff base were synthesized. The yield and physical data of the prepared compound were recorded. The chemical structures were confirmed by using FT-IR, 1H-NMR and elemental analysis CHNS. The antibacterial activity was performed using four microorganisms, two gram positive (S. aureus and B. Cereus) and two gram negative (E. coli and P. Aeroginosa) bacteria and disc diffusion method was used. Tow concentrations from the compound and the standard drug were used (0.010 and 0.005 g/ml). The results were compared to standard drug, cefuroxime. Compound 5f showed the highest activity, while 5b was the least.