A new series of binary and mixed ligand complexesof Ni (II), Cu (II), and Zn (II) with phosphonoformic acid (PFA) a base ligand are studied. The structure of these complexes was identified and confirmed by elemental analysis, molar conductivity, FT-IR and magnetic susceptibility. PFA and their derivatives play an important role in biological processes. It is potential chelating agent and a very active in controlling HIV diseases. Complexes of transition metals ions Ni, Cu, and Zn with primary ligand PFA and secondary ligands (alanine, phenyl-alanine, ethylenediamine, 1, 10-penanthroline, oxalic acid and malonic acid) were carried out and tested for biological activity on Eleucine Coracana (ragi), anti-microbial activity on Staphylococcus-aureus, Bacillus subtilis, Escherichia coli and klebsiella pneumoniae.