The poly ortho toludine was synthesized by oxidative chemical polymerization method in the absence and in the presence of ultrasonic irradiation using potassium dichromate as an oxidant and hydrochloric acid as a dopant. The synthesized polymers were characterized using, UV, IR, TGA and DTA. The Dc conductivity of the polymers and their biological applications like antibacterial and cytotoxic effects were studied. The study revealed that the polymer prepared was conducting in nature and the value was found to be μ2.x10-2 S/cm at 400 C and they are thermally stable even up to 8400C. The antibacterial study was carried by Agar well cut diffusion method and the cytotoxic effect was carried out by MTT assay .The studies indicate that the activities were found to increase with increase in concentrations. The study also revealed that the polymer prepared in the presence of ultrasonic irradiation showed better results even at lower concentrations.