Novel Biginelli dihydropyrimidines were prepared using 4-fluoro nitrobenzene and Piperidine in presence of K2CO3 in DMSO solvent, followed by reduction with Raney Ni and hydrazine hydrate. Then, this amine was reacted with ethyl acetoacetate in Xylene at high temperature and isolated 3-oxo-N-(4-(piperidin-1-yl) phenyl) butanamide as a scaffold, This scaffold was finally react with urea and different aldehyde in presence of p-toluene sulphonic acid as an efficient catalyst to isolate titled compound derivatives. The chemical structures of synthesized compounds were confirmed by 1H-NMR, FT-IR and Mass spectral analysis. The synthesized compounds were screened for potential Antimicrobial, Antifungal and Antimalerial activity.