The new lanthanide (III) complexes of Cefepime, [Ln(Cefepime)(H2O)4](NO3)3, where (Ln=La, Pr, Nd, Sm, Gd and Ho (III)), have been synthesized. The metal complexes were characterized on the basis of elemental analysis, UV-Visible Spectra and FTIR. The spectral data revealed that the Cefepime behaves in tridentate fashion coordinating through carbonyl oxygen of amide and lactum, and carboxylate oxygen. Cefepime and all the complexes were screened for their antimicrobial study against Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes using the disc diffusion method. An enhancement of antimicrobial activity of the Cefepime was observed on complexation.