Condensation of amino pyridines with 3-acetyl-4-hydroxy-2H-chromen-2-one gave 4-hydroxy-3-(1- (pyridinylimino)ethyl)-2H-chromen-2-oneand 4-hydroxy-3-(1-(methylpyridine-2-ylimino)ethyl)-2H-chromen-2-one. These are characterized by IR, 1HNMR, 13CNMR and mass spectral analysis. In vitro biological screening effects of the investigated compounds were tested against fungal species Aspergillusniger, Penicilliumchrysogenumand Aspergillusflavus by the poison plate method.