The copolymers from different feed composition of 2-(N-phthalimido)ethyl acrylate (NPEA) and 1-vinyl-2- pyrolidone (NVP) were synthesized using free radical polymerization using N,N’-dimethylformamide (DMF) as a solvent at 70±1ºC using 2,2’-azobisisobutyronitrile (AIBN) as initiator. The monomer was characterized by 1HNMR and IR spectroscopic techniques. The copolymers were characterized by IR-spectroscopy. The copolymers composition were determined using ultraviolet (UV) spectroscopy. Reactivity ratio for NPEA and NVP was determined by using Fineman-Ross, and Kelen-Tudos methods. The polydispersities of copolymers suggest a strong tendency for chain termination by disproportionation. The thermal stability of the copolymers increases with increase in NPEA content. Antimicrobial activity of the homopolymer and copolymers were also investigated for various microorganisms such as bacteria, fungi and yeast.