2,5 Di-substituted 1,3,4-oxadiazole derivatives was synthesized by the reaction between benzoylchloride and various chlolro, nitro-benzoylchloride and semicarbazide to synthesized (C1-C7) compounds. These compounds were tested for their anti-inflammatory activity determined by rat paw oedema method. All the synthesized compounds have been characterized by 1H NMR, IR and Mass spectral data. The compounds were purified by column chromatography. All synthesized derivatives were determined by the carrageenan induced rat paw oedema model for Anti-inflammatory activity. The entire compound gives good response for anti-inflammatory activity for this activity indomethacine was used as standard drug and compared to new synthesized drugs. Some New Synthesized drugs have to shown better activities for anti-inflammatory.