A Series of 4-methyl-3-[5-(substituted phenyl)-4, 5-dihydro-1H-Pyrazol-3-yl] cinnoline-6-Sulfonamide were synthesized from 4-methyl-3-acetylcinnoline-6-Sulfonamido chalcones and hydrazines. The structure of the synthesized compounds were characterized by UV, IR, NMR & Mass spectral data, and evaluated for their in vitro anti-tubercular and anti-fungal activity. Compound CN-5a was most potent against Mycobacterium tuberculosis H37Rv, and all the analogues showed good anti-fungal activity against various pathogenic fungi.