In the present study a series of novel 2,4-disubsti tuted-[1,3]-thiazole derivatives were synthesized b y the reaction of 2-chloro-6-fluoro/2-fluorobenzaldehyde thiosemicarb azone with phenacyl bromide. 2-chloro-6-fluoro/2- fluorobenzaldehyde thiosemicarbazone was obtained b y treating aldehyde thiosemicarbazone with thiosemicarbazide in the presence acetic acid in et hanol under reflux. Structures of newly synthesized compounds were characterized by spectral studies. New compoun ds were screened for their antimicrobial study and cytotoxic study. The results revealed that many of the synthe sized thiazoles have good cytotoxicity and antimicr obial activity.