As part of ongoing studies in developing new antiviral agents against Bluetongue Virus, a series of novel 1,3,4- oxadiazolyl selenopheno[2,3-d]pyrimidines bearing a choice of aromatic moieties with different substituents were synthesized in five steps reaction sequence with simple protocols. The newly synthesized compounds were characterized by NMR, LC–MS and IR analyses. All the compounds evaluated for their antiviral activity against Bluetoungue Virus (BTV) in Baby Hamster Kidney (BHK–21) cell lines by cell cytopathic effects. The activity data revealed that the compounds 5a, 5b, 5d and 5i evoked a marked antiviral effect and 5b and 5d have shown CC50 value at 3.90μg/ml against BTV.