The metal Tb (III), chlorosubstituted pyrazoles and their complex were used to study the plant growth regulators on spinach. The spinach has high nutrinal and medicinal value. The growth parameters like germination, survival, seedling height and root/shoot ratio etc were studied on the seeds of spinach at different pH. The results found were used to assay the effect of complex, metal Tb (III), chlorosubstituted pyrazoles as ligand and control solution on Spinacia oleracea and the chlorosubstituted pyrazoles and pyrazolines compounds were tested against pathogenic bacteria and studied their effect.