The paper adopted A/O-MBR pilot system as the study object, got a lot of data of the inlet or outlet water quality through water quality monitoring pilot study. With the sample data, this paper established the water quality prediction model of A/O-MBR pilot system by software MATLAB7.1. The water quality prediction model can effectively predict the sewage treatment system dynamic and achieve real-time control, ensured that the system was sustainable, stable and efficient operation.