All of the human activities undergo a series of chemical changes in the body. So the sports biochemical reveals the essence of human life activities in sports activities, can be more scientifically carry out physical education and sports training. Exercise biochemistry is a young discipline, plays an important role in theory and reality in the human sports. Through the application of biochemical technology in sports, can greatly accelerate the development of sports science, promote people’s physical health, and improve the competitive ability and level of the athletes.