Carrot (Daucus carota L.) is one of the valuable vegetables belonging to Umbelliferae plant family and being greatly cultivated in Iran. Our previous study on carrot samples taken from different climatic regions of showed that; some weather changes could affect the carotenoids levels in carrot. Since carotenoids levels in carrot may have influence on the antioxidant activity of the plant; therefore the present study was aimed to determine the maximum free scavenging activity of carrot among Iranian carrot growing regions. Furthermore we studied the association between cartotenoids levels and scavenging activity in carrot. The samples were extracted with 60 % acetone and centrifuged and then the aqueous extracts were evaluated using DPPH (2, 2 - diphenyl - 1- picrylhydrazyl) for free scavenging activities. IC50 of samples were finally compared to ascorbic acid. The highest and lowest inhibition of DPPH radical was found to be 47.20 ± 10.42 and 263.86 ± 39.53 which were related to carrots taken from Zarinshahr and Andimeshk regions in Isfahan and Khuzestan provinces, respectively. IC50 values obtained from these regions were ranged from 25.95 to 337.26. Strong scavenging activity of DPPH radicals was seen in carrots taken from Isfahan province with IC50 value of 1.20 times of Vit-C`s. There was not any significant correlation between carotenoid levels and scavenging activity. Based on this study we conclude that carrots from Zarinshahr in Isfahan have better antioxidant activity compared to other regions of Iran and its carotenoids levels could not play a prominent role in this property.