Because of its high concentration of toxic and complicated composition, the coke plant wastewater is very difficult to meet the discharge standards by normal activated sludge process in practical production. A bioaugmentation technology was applied in biofilm startup of bio-filter and bio-fluidized bed (BF-BFB) system to improve membrane performance and enhance the treatment effect. As a new bacteria cultivated, comamonas testosterone has better synergism and higher COD & NH3-N removal rate. Based on experiments, the system`s rapid startup and stable performance were investigated in this paper. The results showed that the biofilm was mature and stable in 25 days and the removal efficiency of COD and NH3-N were up to more than 95%. By SEM and gene library technology, the dominant strains were identified. The Proteobacteria was the largest bacteria group, accounting for 55.00% of the total library. A stable micro ecosystem formed ultimately to improve the removal effect of recalcitrant organic compounds from industrial coke plant wastewater.