Digera muricata (L.) Mart (Amaranthaceae), commonly known as ‘Latmahuria’ is traditionally used medicinal plant. Laboratory evolutions were made to assess the quantification of primary metabolites in Digera muricata. It contains higher soluble sugars in roots, starch, protein and lipids in leaves and phenols in roots as compared to other parts of the plant. Results indicate that methanolic extracts of plant parts showed significant antimicrobial activity. The leaf extract was found to have maximum activity index against Escherichia coli and Fusarium oxysporum when tested by the disc diffusion method. Aspergillus niger showed significant activity against root extract.