The current work focuses on investigating the effect of the different operational parameters on the removal of Acid Orange 7 (AO7) dye from textile wastewater using natural adsorbent (untreated sugarcane bagasse) and study of the adsorption kinetics involved. The effects of operational parameters such as pH, the amount of biosorbent, initial dye concentration, rotations per minute and temperature were examined. The kinetic study shows that the process under investigation follows the Langmuir adsorption isotherm more proficiently than the Freundlich isotherm. Further investigation of the sorption process was done using the UV-Vis spectra, Fourier Transform Infrared (FTIR) spectroscopy and X-ray Diffraction (XRD). Out of several chosen parameters, the optimum conditions for efficient dye removal was found to be the dose of biosorbent (8 g), initial dye concentration (4 mg/100mL), initial pH (2.5) and temperature (30°C). This combination gave a reduction of O.D. (measured at 485 nm) by 85.42%. From the investigation sugarcane bagasse proved good biosorbent for the AO7 dye.